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Environmental DNA (eDNA) analysis is a technique used to identify and study the DNA of organisms present in a particular environment without directly observing them. The process begins with the collection of samples using a sampling kit, consisting of plastic syringes and filters in the case of water. Once the sample is collected, DNA extraction is performed to isolate and purify the DNA from the environmental matrix. Next, high-throughput sequencing reads out the DNA information. The resulting data is then analyzed using bioinformatics software to identify the species present in the sample. Finally, a report is generated to summarize the findings and provide information on the biodiversity of the sampled environment.


In principle, samples may be taken from water, soil, or even air. DNA is everywhere and new methods to detect it are being developed. Water, particularly freshwater, is the most established starting point for eDNA analysis. Currently, we are offering the following services:

Fish & Amphibian eDNA Service

At SimplexDNA®, we are committed to providing accurate, reliable and affordable eDNA services to help you uncover the biodiversity of aquatic environments. Our state-of-the-art technology and expertise enable us to provide you with comprehensive insights into the environmental conditions and species diversity of freshwater habitats.

Starting from a filtered water sample, we carry out eDNA metabarcoding and bioinformatic analysis to generate taxonomic reports according to your requirements. We offer a flexible service from quoting over sampling to final data delivery.


  • Sample preparation and library PCR

  • Sequencing of the 12S/16S gene

  • Analysis of defined taxonomic groups

  • Discover presence or absence of species

  • Qualitative and quantitative information

Fish Swimming

Successful Showcase Projects





Canton Aargau / Canton Bern / Canton Solothur

"Monitoring of non-native species using eDNA as part of the coordinated biological survey of the Aare river"

Canton Zug

"eDNA survey of fish diversity for the Zug Fish Atlas"

Canton Aargau

"Impact monitoring of watercourse revitalization: eDNA survey of fishes"

Canton Aargau

"Monitoring of non-native speices using eDNA in Lake Hallwil"

Tree of Life eDNA Service 


Introducing SimplexDNA's Tree of Life eDNA Service - the most comprehensive snapshot of an ecosystem yet. Our innovative service detects biodiversity across the evolutionary tree, from insects and fungi to bacteria, archaea, plants, birds, and mammals.

By sampling the environmental DNA present in soil, water, or air samples, we can identify thousands of species present in any ecosystem. With our Tree of Life eDNA Service, you'll receive a detailed report of the ecosystem's biodiversity, allowing you to gain insight and manage the habitat.


  • Early access service

  • Assess the biodiversity of an ecosystem

  • Define taxonomic groups for more detail

Custom eDNA Service

As a small expert lab, we are perfectly positioned to accommodate your custom DNA meta-barcoding project. Our offer can be tailored from general community assessment of animals, fungi, plants and microorganisms to single species detection. We can work with water, soil and other sample formats and will work with you to develop suitable methods.

Available Services

  • Sampling design and planning

  • Sampling kits and sampling training

  • DNA extraction

  • Amplicon library preparation

  • Sequencing of 16S, 12S, CO1 and ITS genes

  • Custom bioinformatic analyses

  • qPCR Single Species Detection

  • Pathogen Detection

  • Research and Development

Pipette Inserted Into Test Tube
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